Hesperidin extraction from orange peel
2024-03-21 17:34:44
How to extract hesperidin
The extraction methods of hesperidin include solvent extraction, alkaline extraction and acid precipitation, carbon powder adsorption, and ion exchange. Among them, the alkaline extraction and acid precipitation method has simple operation, low cost and high extraction rate. Orange peel is soluble in dilute alkali and pyridine and hot water above 70℃, slightly soluble in methanol, and almost insoluble in acetone, benzene and chloroform. The extraction of hesperidin mainly utilizes the two phenolic hydroxyl groups contained in it to react with sodium ions in the solution under alkaline conditions to form sodium salt and dissolve it, and then acidify and cool to make it precipitate out of the solution. The extraction of hesperidin from citrus peel generally adopts hot extraction method and soaking extraction method, and the yield is not ideal. In recent years, ultrasonic extraction of active ingredients from natural plants (especially Chinese herbal medicines) has been extensively carried out, and certain progress has been made. Use hesperidin for extraction under alkaline conditions by ring-opening dissolution, and separation under acidic conditions by closed-loop precipitation. Increasing the amount of alkali during extraction can reduce the amount of ethanol, but the alkalinity should not be too large, otherwise, hesperidin is easy to be oxidized destroy.
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